Childhood Cancer Repository, powered by Alex's Lemonade Stand Foundation

A Children's Oncology Group Resource Laboratory

Requesting Cell-lines

List of All Available Lines

How to Request a Cell Line:

(1) Select the cell line(s) by clicking each check box next to the cell line name.
(2) Fill out the institutional or commercial/industry information at the bottom of the page.
(3) Attach a fully signed MTA (including Attachment A)
(4) Attach any additional documents required for your request (i.e. a justification for any request over 5 cell lines total)
(5) Click Submit.

For all other questions regarding your request and/or MTA, please visit our FAQ page.

To view a complete cell line description (origin, growth conditions, pictures, etc.), click on the cell line name to open a pdf data sheet to view and/or download.

All cell lines were established in 20% O2 unless otherwise noted. Cell lines ending in "h" designates cell line was established in hypoxic conditions (5% O2 tension).

Please visit the CCcells.org website frequently, as cell line data is continually being updated and new cell lines are added.

Brain Tumor Lines
Request	Cell Line	Disease	Diagnosis	Gender	Age (Years)	Phase of Therapy¹	Site of specimen	DT⁵ (hours)	Data Sheet
	BT-12^*	Brain Tumor	Atypical teratoid/rhabdoid	F	0.2	Dx
	CHLA-200	Brain Tumor	Glioblastoma multiforme	M	12	PD-PM	Parietal lobe	54
	CHLA-259	Brain Tumor	Anaplastic medullablastoma	M	14	Dx	Posterior fossa	76
	CHLA-266^*	Brain Tumor	Atypical teratoid/rhabdoid	F	1.5	DX	posterior fossa	45
	SJ-GBM2^*	Brain Tumor	Glioblastoma multiforme	F	5	PD	brain

Ewing's Family of Tumors Lines
Request	Cell Line	Disease	Diagnosis	Age (Years)	Phase of Therapy¹	EWS/FLI1 Status⁴	p53 Status³	DT⁵ (hours)	Data Sheet
	CB-AGPN	Ewing's Family of Tumors	PNET
	CHLA-9^*	Ewing's Family of Tumors	PNET	14	Dx	FLI1	F	51
	CHLA-10^*	Ewing's Family of Tumors	PNET	14	PD	FLI1	N	32
	CHLA-25**	Ewing's Family of Tumors	PNET	2.6	PD	ERG	N	99
	CHLA-32	Ewing's Family of Tumors	PNET	8.5	Dx	FLI1	F	26
	CHLA-57	Ewing's Family of Tumors	PNET
	CHLA-99	Ewing's Family of Tumors	Ewing's Sarcoma
	CHLA-218	Ewing's Family of Tumors	PNET		PD
	CHLA-258^*	Ewing's Family of Tumors	PNET	14	PD	FLI1	N	89
	COG-E-352	Ewing's Family of Tumors	PNET	17	PD-PM	ERG	N	28
	TC-32	Ewing's Family of Tumors	PNET	17	Dx	FLI1	N	24
	TC-71^*	Ewing's Family of Tumors	Ewing's Sarcoma	22	PD	FLI1	N	21
	TC-106	Ewing's Family of Tumors	Ewing's Sarcoma	19	Dx		F
	TC-138	Ewing's Family of Tumors				FLI1
	TC-205	Ewing's Family of Tumors				FEV

Leukemia Lines
Request	Cell Line	Disease	Diagnosis	Gender	Age (Years)	Phase of Therapy¹	Site of Specimen	DT⁵ (hours)
	COG-LL-317^*	Leukemia	T-cell ALL	M	1.7	PD	Bone marrow	30-40
	COG-LL-317h^*	Leukemia	T-cell ALL	M	1.7	PD	Bone marrow	19-20
	COG-LL-319h	Leukemia	Pre-B ALL	F	13	Dx	Bone marrow	50-62
	COG-LL-329h	Leukemia	T-cell ALL			Dx	Bone marrow
	COG-LL-332h	Leukemia	T-cell ALL	M	10	PD	Bone marrow	30-34
	COG-LL-355h	Leukemia	Pre-B ALL			Dx	Bone marrow
	COG-LL-356h	Leukemia	Pre-B ALL			Dx	Bone marrow
	COG-LL-384h	Leukemia	T-cell ALL			PD	Bone marrow

Neuroblastoma Lines
Request	Cell Line	Disease	Stage	Age (Months)	Phase of Therapy¹	MYCN Status²	p53 Status³	DT⁵ (hours)	Data Sheet
	CHLA-15	Neuroblastoma	4	18	DX	N	F	21
	CHLA-20	Neuroblastoma	4	18	PD	N	F	28
	CHLA-42	Neuroblastoma	4	13	Dx	N	F
	CHLA-90	Neuroblastoma	4	102	PD-BMT	N	N	59
	CHLA-95	Neuroblastoma	4		PD	A
	CHLA-122	Neuroblastoma	4	24	Dx	A	F	72
	CHLA-136	Neuroblastoma	4	36	PD-BMT	A	F	44
	CHLA-140	Neuroblastoma	4		PD	N
	CHLA-171	Neuroblastoma	4	101	PD-PM	N	N
	LA-N-5	Neuroblastoma		5	Dx	A		100
	LA-N-6	Neuroblastoma	4	60	PD	N	F	150
	NB-1643^*	Neuroblastoma	4	< 36	Dx	A
	NB-EBc1^*	Neuroblastoma	4	< 36	PD	N
	SK-N-BE(1)	Neuroblastoma	4	24	Dx	A	F	96
	SK-N-BE(2)	Neuroblastoma	4	24	PD	A	N	27
	SK-N-FI	Neuroblastoma		132	PD	N
	SMS-KAN	Neuroblastoma	4	36	Dx	A	F	95
	SMS-KANR	Neuroblastoma	4	36	PD	A	F	69
	SMS-KCN	Neuroblastoma	4	11	Dx	A	F	109
	SMS-KCNR	Neuroblastoma	4	11	PD	A	F	72
	SMS-LHN	Neuroblastoma	4	24	PD	N	F	216
	SMS-SAN	Neuroblastoma	4	36	Dx	A	F	71
	COG-N-291	Neuroblastoma			PD	N	N	224
	CHLA-53	Neuroblastoma	4		PD-PM	A		92
	CHLA-61	Neuroblastoma	4	168	PD-PM	N	F	102
	CHLA-11	Neuroblastoma	4		PD-BMT,PM	A	F	53
	CHLA-12	Neuroblastoma	4		PD-BMT,PM	A		65
	CHLA-52	Neuroblastoma	4		PD-PM	A	F	88
	CHLA-60	Neuroblastoma	4	168	PD-PM	N	F	96
	CHLA-79	Neuroblastoma	4	24	PD-BMT	N	F
	CHLA-144	Neuroblastoma	4		Dx	A
	CHLA-145	Neuroblastoma	4		PD	A
	CHLA-172	Neuroblastoma	4		PD-BMT	N	N
	CHLA-225	Neuroblastoma			PD-PM	A	N	62
	CHLA-247	Neuroblastoma			PD	A
	CHLA-253	Neuroblastoma			PD	A
	COG-N-316	Neuroblastoma	4	62	Dx	A
	COG-N-321	Neuroblastoma	3	18	PD	A	N
	COG-N-347	Neuroblastoma	4	24	Dx	A		126
	COG-N-399	Neuroblastoma	4	9.6	PD-PM	A	F	40
	COG-N-415	Neuroblastoma	4	24	PD	A	N	50
	COG-N-421	Neuroblastoma	4	119	PD-PM	A		65
	COG-N-440	Neuroblastoma	4	18	PD-PM	A		65
	COG-N-453	Neuroblastoma	4	60	PD-PM	A		71
	COG-N-496	Neuroblastoma	4	38	Dx	A		85
	SK-N-RA	Neuroblastoma			PD		F

Rhabdomyosarcoma Lines
Request	Cell Line	Disease	Diagnosis	Age (Years)	Phase of Therapy¹	p53 Status³	Site of specimen	DT⁵ (hours)	Data Sheet
	Rh18^*	Rhabdomyosarcoma	embryonal	2	Dx	N	perineum	65
	Rh30^*	Rhabdomyosarcoma	alveolar	17	Dx	F	bone marrow	35
	Rh41^*	Rhabdomyosarcoma	alveolar		PD	N	lung	60

¹ - Phase of therapy for the patient at the time the specimen used to derive the cell line was obtained
Dx (Diagnosis): cell lines derived from samples of patients prior to treatment
PD (Progressive Disease): cell lines derived from samples of patients who relapsed after chemotherapy
PD-BMT: cell lines derived from samples of patients who relapsed after chemotherapy and bone marrow transplantation
PD-PM: cell lines derived from samples of post-mortem patients who relapsed after chemotherapy

² - N-Myc oncogene amplification
N: MYCN Non-Amplified
A: MYCN Amplified

³ - p53 gene function
F: Functional
N: Non-Functional

⁴ - EWS/FLI1 Status
FLI1: friend leukemia insertion - chromosome 11, t(11;22)
ERG: ets-related gene - chromosome 21, t(21;22)

⁵ - Doubling Time

* - NCI PPTP Cell Lines

** - CHLA-25 (May et al) and TTC-466 (Sorensen et al) were established in two separate laboratories, but from the same specimen obtained from a single biopsy from one patient.

For details on all the NCI PPTP cell lines see:

Kang MH, Smith MA, Morton CL, Nino Keshelava N, Houghton PJ, Reynolds CP: National Cancer Institute Pediatric Preclinical Testing Program: Model description for in vitro cytotoxicity testing. Pediatr Blood Cancer 56:239-49, 2011.

Shipping and Contact Information
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If you are requesting more than 5 cell lines: Who should review the proposal? (Please select one)	The Disease Committee (for requests of material of a single disease) The Biology and Translational Research Committee (for requests of material from multiple diseases)
If you are requesting 5-15 cell lines, upload a < 1 page description of research and justification for the lines. (PDF format only; maximum allowed file size is 2 megabytes)
If you are requesting more than 15 lines, upload a full COG biology specimen application form. (PDF format only; maximum allowed file size is 2 megabytes)
Upload your COG Specimen Transfer Agreement: (PDF format only; maximum allowed file size is 2 megabytes)	You can also FAX this form to 806-743-2691 if you have difficulty uploading it.
Do you have any special requests or comments? If so, please enter them here.

Download Open Distribution List MTAs:

Print the MTA form, fill in your request, sign it, and either scan it to PDF format for sending to us (using the upload button on our online cell request form), or fax it to 806-743-2691.

Please contact us if you are requesting cell lines that are not currently on the Open Distribution List.

Investigators may request up to 5 cell lines (cumulative overall requests) with minimal project descriptions (to be briefly outlined in Attachment A of the MTA).

Requests between 5 and 15 lines requiring short justification and local review and requests exceeding 15 lines will require a formal application and approval by the appropriate COG committee.

The prioritization of how material is distributed will be determined by the COG Disease Committees and the COG Biology and Translational Research Committee.

Some previously published cell lines, with originating investigator approval, will be characterized by the Childhood Cancer Repository laboratories and made available through this website.

Questions about the Childhood Cancer Repository can be directed to us here.

Childhood Cancer Repository, powered by Alex's Lemonade Stand Foundation

A Children's Oncology Group Resource Laboratory

Requesting Cell-lines

List of All Available Lines

Brain Tumor Lines

Ewing's Family of Tumors Lines

Leukemia Lines

Neuroblastoma Lines

Rhabdomyosarcoma Lines

Shipping and Contact Information