Neuroblastoma PDXs OPEN

Request	PDX	Phase of Therapy	Sample Type	Age (yrs)	Sex	MYCN	TH mRNA	GD2	TMM	HST	WES	RNA Seq		IDEXX	Cell Line (PDCL)	Lag time Days	Doubling time Days
												PDX	PDCL
	COG-N-415x	PD-PM	Blood	1.4	F	+	+	+	TERT	✓	✓	✓	✓	✓	✓	44	5
	COG-N-421x	PD-PM	Blood	2.7	M	+	+	+	TERT	✓	✓	✓	✓	✓	✓	41	6
	COG-N-424x	Dx	Tumor	0.5	M	+	+	+	TERT	✓	✓	✓		✓		26	8
	COG-N-440x	PD-PM	Blood	0.9	F	+	+	+	TERT	✓	✓	✓	✓	✓	✓	40	5
	COG-N-452x	PD-PM	Blood	1.4	M	+	+	+	TERT	✓	✓	✓		✓	✓	39	4
	COG-N-453x	PD-PM	Tumor	1.4	M	+	+	+	TERT	✓	✓	✓	✓	✓	✓	44	8
	COG-N-470x	PD-PM	Blood	1.6	F	-	+	+	TERT	✓	✓	✓	✓	✓	✓	76	8
	COG-N-471x	PD-PM	Blood	2.6	F	+	+	+	TERT	✓	✓	✓	✓	✓	✓	50	7
	COG-N-480x	Dx	BM	1.0	M	+	+	+	EST	✓	✓	✓	✓	✓	✓	34	4
	COG-N-496x	Dx	BM	3.2	M	+	+		TERT	✓	✓	✓	✓	✓	✓	95	14
	COG-N-518h	Dx	BM	3.3	F	-	+		UNK	✓	✓	✓		✓	✓
	COG-N-519x	PD-PM	Blood	2.0	M	+	+	+	TERT	✓	✓	✓	✓	✓	✓	27	3
	COG-N-546x	Dx	BM	1.3	F	+	+	+	TERT	✓	✓	✓	✓	✓	✓	79	14
	COG-N-549x	PD	Tumor	1.2	M	-	+	+	TERT	✓	✓	✓	✓	✓	✓	73	13
	COG-N-557x	Dx	Tumor	2.7	M	+	+	+	TERT	✓	✓	✓	✓	✓	✓	137	17
	COG-N-560x	Dx	Tumor	1.0	M	+	+	+	TERT	✓	✓	✓		✓
	COG-N-561x	PD-PM	Blood	1.6	F	+	+	+	TERT	✓	✓	✓	✓	✓	✓	57	9
	COG-N-564x	PD-PM	Blood	NA	F	+	+	+	TERT	✓	✓	✓	✓	✓	✓	32	3
	COG-N-573x	Dx	BM	2.3	M	+	+	+	TERT	✓	✓	✓	✓	✓	✓	25	6
	COG-N-579x	PD	BM	5.5	F	-	+	+	TERT	✓	✓	✓	✓	✓	✓	54	5
	COG-N-590x	Dx	Tumor	4.6	M	+	+	+	TERT	✓	✓	✓	✓	✓	✓	54	7
	COG-N-603x	Dx	Tumor	0.7	M	+	+	+	TERT	✓	✓	✓	✓	✓	✓	29	6
	COG-N-615x	PD-PM	Blood	NA	M	-	+	+	TERT	✓	✓	✓	✓	✓	✓	85	10
	COG-N-618x	PD	BM	2.7	M	-	+	+	TERT	✓	✓	✓	✓	✓	✓	65	13
	COG-N-619x	PD	BM	2.7	M	-	+	+	TERT	✓	✓	✓	✓	✓	✓	65	17
	COG-N-623x	PD	Tumor	0.7	M	+	+	+	TERT	✓	✓	✓	✓	✓	✓	47	8
	COG-N-625x	PD	Tumor	5.0	M	-	+	+	ALT	✓	✓	✓		✓	✓	105	9
	COG-N-628x	Dx	BM	2.4	M	+	+	+	TERT	✓	✓	✓	✓	✓	✓	79	15
	COG-N-636x	Dx	Tumor	2.0	M	+	+		TERT	✓	✓	✓	✓		✓	50	10
	COG-N-653x	Dx	Tumor	1.7	M	+	+	-	TERT	✓	✓	✓	✓		✓	83	12
	COG-N-660x	PD-PM	Blood	NA	M	-	+	+	TERT	✓	✓	✓		✓	✓	63	9
	COG-N-669x	PD-PM	Blood	NA	F	-	+	-	ALT	✓	✓	✓		✓
	COG-N-672x	PD-PM	Blood	1.7	M	+	+	+	TERT	✓	✓	✓		✓	✓	20	3
	COG-N-676x	PD	Tumor	4.9	M	-	+		TERT	✓	✓	✓
	COG-N-677x	PD	BM	3.9	M	+	+	+	TERT	✓	✓	✓			✓	88	12
	COG-N-682x	Dx	BM	1.1	F	+	+	+	TERT	✓	✓	✓	✓		✓	21	6
	COG-N-683x	DX	BM	1.1	F	+	+	+	TERT	✓	✓	✓	✓		✓	29	12
	COG-N-685x	PD-PM	Blood	NA	F	+	+	+	TERT	✓	✓	✓	✓	✓	✓	60	6
	COG-N-697x	Dx	Tumor	2.6	F	+	+	+	TERT	✓	✓	✓	✓		✓	88	16
	COG-N-700x	Dx	BM	6.3	F	-	+	+	ALT	✓	✓	✓	✓	✓	✓	102	10
	COG-N-707x	PD	BM	NA	M	+		+	TERT	✓	✓				✓	59	7
	COG-N-708x	Dx	Tumor	2.3	F	-		+	TERT	✓	✓	✓	✓		✓	62	8
	COG-N-709x	Dx	Tumor	2.0	M	-		+	TERT	✓	✓		✓		✓	76	20
	COG-N-718x	Dx	Tumor	6.6	F	+		+	TERT	✓	✓				✓	68	13
	COG-N-719x	PD	Tumor	2.3	M	+	+	+	TERT	✓	✓		✓		✓	45	13
	COG-N-731x	PD	BM	10.3	M	-	-	+	TERT	✓	✓				✓	36	14
	COG-N-732x	Dx	Tumor	4.3	F	+		+	TERT	✓					✓	51	7
	COG-N-736x	Dx	Tumor	2.6	F	+		+	TERT	✓	✓				✓	64	10
	COG-N-740x	Dx	Tumor	2.0	M	+	+	+	TERT	✓					✓	70	7
	COG-N-743x	Dx	Tumor	0.9	F	+		+	TERT	✓					✓	80	5
	COG-N-781x	PD-PM	Blood	2.6	M	-		+	TERT	✓
	COG-N-789x	Dx	Tumor	1.9	M	-		+		✓
	COG-N-791x	Dx	Tumor	3.8	M	+	+	+	TERT	✓					✓	86	9
	COG-N-804x	Dx	Tumor	3.3	M	-		+		✓
	COG-N-809x	Dx	BM	NA	F	-		-		✓					✓	33	7
	COG-N-813x	PD-PM	blood	NA	M	-		+	TERT	✓					✓	36	7
	COG-N-815x	PD-PM	blood	2.4	M	+		+	TERT	✓					✓	32	6
	COG-N-817x	Dx	BM	3.4	F	-		+	TERT	✓
	COG-N-818x	PD	Tumor	2.3	F	-		+		✓					✓	102	13
	COG-N-835x	PD	BM	1.3	F	+		+		✓					✓	41	8
	COG-N-845x	Dx	BM	NA	M	+	+		TERT	✓					✓	167	10
	COG-N-853x	PD-PM	Blood	1.6	F	+	+	+	TERT	✓					✓	65	6
	COG-N-854x	Dx	Tumor	0.4	M	+		+		✓					✓	46	5
	Felix-PDX	PD-PM	Blood	4.0	M	-	+	+	TERT	✓	✓	✓	✓	✓	✓	59	7

¹ - Phase of therapy for the patient at the time the specimen used to derive the cell line was obtained
Dx (Diagnosis): cell lines derived from samples of patients prior to treatment
PD (Progressive Disease): cell lines derived from samples of patients who relapsed after chemotherapy
PD-BMT: cell lines derived from samples of patients who relapsed after chemotherapy and bone marrow transplantation
PD-PM: cell lines derived from samples of post-mortem patients who relapsed after chemotherapy

² -MYCN oncogene amplification
N: MYCN Non-Amplified
A: MYCN Amplified

³ - TP53 gene function
F: Functional
N: Non-Functional

⁴ - EWS/FLI1 Status
FLI1: friend leukemia insertion - chromosome 11, t(11;22)
ERG: ets-related gene - chromosome 21, t(21;22)

ALK mutation

WT: Wild Type

TMM:Telomere Maintenance mechanism

✓ = Available/available upon request

SQ- subcutaneous injection

TV- intravenous (tail vein)

EST- ever shorter telomeres, i.e. telomerase and ALT negative

TERT+- telomerase positive

ALT - alternate lengthening of telomeres, C-circle positive